In vitro priming to direct neuronal fate in adult neural progenitor cells

作者：Vazey Elena M; Connor Bronwen^*

来源：Experimental Neurology, 2009, 216(2): 520-524.

DOI：10.1016/j.expneurol.2008.12.023

• Adult neural progenitor cells
• Sub-ventricular zone
• Lithium chloride
• Priming
• Cell transplantation

We have previously reported methods that allow for the routine isolation, culture and transplantation of multipotent neural progenitor cells (NPCs) derived from the adult rat sub-ventricular zone (SVZ). In order to expand these techniques, the aim of the current study was to develop an in vitro strategy which can "prime" proliferating adult NPCs towards a neuronal fate during their subsequent differentiation under standard conditions. Our results show that transient exposure to lithium chloride during in vitro proliferation of adult SVZ-derived NPCs has the ability to alter differential fate in vitro and increase the proportion of cells expressing neuronal markers while at the same time causing a reduction in glial progeny. Treatment of adult NPCs with lithium chloride may therefore provide a novel mechanism by which adult NPCs can be primed towards a specific neuronal fate for cell replacement therapy.

• 出版日期2009-4