An enzyme-linked immunosorbent assay for the detection of bifenthrin

作者：Li Bo^*; Shi Hai Yan; Wang Ming Hua

来源：Chinese Journal of Analytical Chemistry, 2008, 36(1): 34-38.

• bifenthrin
• polyclonal antibody
• enzyme-linked immunosorbent assay

An indirectly competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for the quantitative detection of bifenthrin. The hapten (LBc, 2-methyl [1,1-biphenyl] -3-methoxy) carbonyl propionic acid) for the bifenthrin was prepared starting from the metabolite of bifenthrin (2-methyl-3-phenylbenzyl alcohol). Then, the hapten (LBc) was conjugated. to bovine serum albumin (BSA) with the carbodiimide method to form immuno-antigen LBc-BSA; the haptens LBc and LBy ((2-methyl[ 1,1-biphenyl]-3-yl) carboxylic acid) was conjugated to ovalbumin (OVA) with mixed anhydride method to form the the coating antigens. The new zealand rabbits were immunized by conjugate of LBc-BSA and titres of anti-bifenthrin serum (5.12 x 10(4)) were determined by non-competitive indirect enzyme-linked assay procedure. A heterologous assay system using the coating antigen format was more sensitive. After optimization of the ic-ELISA conditions, such as pH values, inonic strengths, concentrations of methanol, the pH of 7.5, PBS of 0.3 mol/L Na+, and 30% methanol were selected as optimum assay conditions. The IC50 for bifenthrin was 2.16 +/- 0.32 mg/L, and lower detection limit (LDL) was 0.016 +/- 0.002 mg/L. Pyrethroids, such as cyhalothrin, deltamethrin, cypermethrin, fenvalerate, fenpropathrin and the pyrethroid metabolite 3-phenoxybenzoic acid, did not form the cross-reaction significant in this assay.

• 出版日期2008-1
• 单位南京农业大学