科研之友
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摘要
The majority of pregnancy loss in cattle occurs between Days 8 and 16 of gestation, coincident with the initiation of conceptus elongation and the onset of maternal recognition of pregnancy. Differences in conceptus length on the same day of gestation may be related to an inherent lack of developmental competency or may simply be a consequence of asynchrony with the maternal environment. The objective of this work was to characterize differential patterns of mRNA expression between short and long bovine conceptuses recovered on Day 15 of gestation. Embryos were produced from super-ovulated Holstein donor cows, and groups of Grade-1 and Grade-3 compact morulas were transferred into recipient heifers at Day 6.5 of their cycle. Conceptuses were recovered at Day 15 of gestation, and measured to assess overall length and area. Total RNA was extracted and analyzed on individual GeneChip Bovine Genome Arrays (Affymetrix, Santa Clara, CA). Gene expression was compared between conceptuses derived from the transfer of Grade-1 versus Grade-3 embryos; no differences were identified in the profiles of Day-15 conceptuses of these different embryo grades. When gene expression was compared between conceptuses classified as either short (mean length of 4.2 +/- 0.1mm [standard error]) or long (24.7 +/- 1.9mm) upon recovery at Day 15 of gestation, a total of 348 genes were differentially expressed. Of these, 221 genes were up-regulated and 127 were down-regulated in long compared to short conceptuses. In summary, differences in gene expression were identified between conceptuses recovered on Day 15 of gestation, based on their length. These data may be used to identify genes and cellular pathways involved in enhanced conceptus elongation that could serve as markers of successful pregnancy. Mol. Reprod. Dev. 83: 424-441, 2016.
- 出版日期2016-5
