Acute toxicity and sublethal effects of myclobutanil on respiration, flight and detoxification enzymes in Apis cerana cerana

作者：Han, Wensu; Wang, Yajun; Gao, Jinglin; Wang, Shijie; Zhao, Shan; Liu, Junfeng; Zhong, Yihai; Zhao, Dongxiang^*

来源：Pesticide Biochemistry and Physiology, 2018, 147: 133-138.

DOI：10.1016/j.pestbp.2017.11.001

• Apis cerana cerana
• Respiration
• Flight
• Detoxification enzymes
• Myclobutanil

Myclobutanil is currently used on the flowering plants. Little is known about how Apis cerana cerana respond to myclobutanil exposure. Hence, the acute toxicity of myclobutanil and its sublethal effects on respiration, flight and detoxification enzymes [7-ethoxycoumarin O-deethylase (ECOD) and glutathione S-transferases (GSTs)] in A. cerana cerana were investigated. The results indicated that formulation grade myclobutanil showed moderate toxicity to A. cerana cerana either contact (LD50 = 4.697 mu g/bee) or oral (LD50 = 2.154 mu g/bee) exposure. Sublethal dose of myclobutanil significantly reduced the respiration rate of workers at 24 h and 48 h regardless of the exposure method. However, myclobutanil didn't significantly affect the take-off flight. After nurse bees exposure to the dose (LD5) of formulation-grade myclobutanil, ECOD activity was significantly induced when compared with control, but GST activity didn't change. In the forager bees, no enzyme markers response was obtained in this test. From the present study we can infer that myclobutanil disturb respiration and P450-mediated detoxification of the individual bees of A. cerana cerana. Thus, myclobutanil may has risk for A. cerana cerana, it should be cautiously used.

• 出版日期2018-5
• 单位中国热带农业科学院; 海南大学