A mathematical model for fluid shear-sensitive 3D tissue construct development

作者：Liu Dan^*; Chua Chee Kai; Leong Kah Fai

来源：Biomechanics and Modeling in Mechanobiology, 2013, 12(1): 19-31.

DOI：10.1007/s10237-012-0378-7

• Dynamic culture
• Numerical modeling
• Computational fluid dynamics
• Flow shear
• Perfusion
• Tissue engineering

This research studies dynamic culture for 3D tissue construct development with computational fluid dynamics. It proposes a mathematical model to evaluate the impact of flow rates and flow shear stress on cell growth in 3D constructs under perfusion. The modeling results show that dynamic flow, even at flow rate as low as 0.002 cm/s, can support much better mass exchange, higher cell number, and more even cell and nutrient distribution compared to static culture. Higher flow rate can further improve nutrient supply and mass exchange in the construct, promoting better nutritious environment and cell proliferation compared to lower flow rate. In addition, consideration of flow shear stress predicts much higher cell number in the construct compared to that without shear consideration. While the nutrient can dominate shear stress in influencing cell proliferation, the shear effect increases with flow rate. The proposed model helps tissue engineers better understand the cell-flow relationship at the molecular level during dynamic culture.

• 出版日期2013-1
• 单位南阳理工学院