Atrioventricular Conduction and Arrhythmias at the Initiation of Beating in Embryonic Mouse Hearts

作者：Chen Fuhua; De Diego Carlos; Chang Marvin G; McHarg Jennifer L; John Scott; Klitzner Thomas S; Weiss James N^*

来源：Developmental Dynamics, 2010, 239(7): 1941-1949.

DOI：10.1002/dvdy.22319

• mouse embryo
• cardiac development
• action potential
• AV conduction
• calcium transient
• optical mapping
• arrhythmias

To investigate cardiac physiology at the onset of heart beating in embryonic mouse hearts, we performed optical imaging of membrane potential (Vm) and/or intracellular calcium (Ca(i)). Action potentials and Ca(i) transients were detected in similar to 50% of mouse embryo hearts at E8.5, but in all hearts at E9.0, indicating that beating typically starts between E8-E9. Beating was eliminated by Ca channel blocker nifedipine and the I(f) blocker ZD7288, unaffected by tetrodotoxin and only mildly depressed by disabling sarcoplasmic (SR) and endoplasmic (ER) reticulum Ca cycling. From E8.5 to E10, conduction velocity increased from 0.2-1 mm/s to >5 mm/s in first ventricular and then atrial tissue, while remaining slow in other areas. Arrhythmias included atrioventricular reentry induced by adenosine. In summary, at the onset of beating, I(f)-dependent pacemaking drives both AP propagation and Ca(i) transient generation through activation of voltage-dependent Ca channels. Na channels and intracellular Ca cycling have minor roles. Developmental Dynamics 239:1941-1949, 2010.

• 出版日期2010-7