A new boronic acid fluorescent sensor based on fluorene for monosaccharides at physiological pH

作者：Hosseinzadeh Rahman^*; Mohadjerani Maryam; Pooryousef Mona; Eslami Abbas; Emami Saeed

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2015, 144: 53-60.

DOI：10.1016/j.saa.2015.02.066

• Boronic acid
• Monosaccharides
• Fluorene
• Sensor
• Fluorescence

Fluorescent boronic acids are very useful fluorescent sensor for detection of biologically important saccharides. Herein we synthesized a new fluorene-based fluorescent boronic acid that shows significant fluorescence changes upon addition of saccharides at physiological pH. Upon addition of fructose, sorbitol, glucose, galactose, ribose, and maltose at different concentration to the solution of 7-(dimethylamino)-9,9-dimethy1-9H-fluoren-2-y1-2-boronic acid (7-DMAFBA, 1), significant decreases in fluorescent intensity were observed. It was found that this boronic acid has high affinity (K-a = 3582.88 M-1) and selectivity for fructose over glucose at pH = 7.4. The sensor 1 showed a linear response toward a-fructose in the concentrations ranging from 2.5 x 10(-5) to 4 x 10(-4) MOI L-1 with the detection limit of 1.3 x 10(-5) mol L-1.

• 出版日期2015-6-5