Arcobacter acticola sp nov., isolated from seawater on the East Sea in South Korea

作者：Park Sooyeon; Jung Yong Taek; Kim Sona; Yoon Jung Hoon^*

来源：Journal of Microbiology, 2016, 54(10): 655-659.

DOI：10.1007/s12275-016-6268-4

• Arcobacter acticola
• East Sea
• novel species
• polyphasic taxonomy
• seawater

A Gram-stain-negative, facultative aerobic, non-flagellated, and rod-shaped bacterium, designated AR-13(T), was isolated from a seawater on the East Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain AR-13(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 0-0.5% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain AR-13(T) fell within the Glade comprising the type strains of Arcobacter species, clustering coherently with the type strain of Arcobacter venerupis. Strain AR-13(T) exhibited 16S rRNA gene sequence similarity values of 98.1% to the type strain of A. venerupis and of 93.2-96.9% to the type strains of the other Arcobacter species. Strain AR-13(T) contained MK-6 as the only menaquinone and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:0, C-18:1 omega 7c, and summed feature 2 (iso-C-16:1 I and/or C-14:0 3-OH) as the major fatty acids. The major polar lipids detected in strain AR-13(T) were phosphatidylethanolamine, phosphatidylglycerol, and one unidentified aminophospholipid. The DNA G+C content was 28.3 mol% and its mean DNA-DNA relatedness value with the type strain of A. venerupis was 21%. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain AR-13(T) is separated from recognized Arcobacter species. On the basis of the data presented, strain AR-13(T) is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter acticola T. nov. is proposed. The type strain is AR-13(T) (=KCTC 52212(T) =NBRC 112272(T)).

• 出版日期2016-10