A Human Short Open Reading Frame ( sORF)-encoded Polypeptide That Stimulates DNA End Joining

作者：Slavoff Sarah A; Heo Jinho; Budnik Bogdan A; Hanakahi Leslyn A^*; Saghatelian Alan

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289(16): 10950-10957.

DOI：10.1074/jbc.C113.533968

• DNA Repair
• Peptide Interactions
• Peptides
• Protein-Protein Interactions
• Proteomics
• Short ORF

Background: Large numbers of peptides encoded in human short open reading frames have been recently identified but not yet functionally characterized. Results: A peptide interacts with the Ku heterodimer and stimulates nonhomologous end-joining DNA repair. Conclusion: Newly discovered cellular peptides can be functionally characterized by identifying their interaction partners. Significance: Short ORF-encoded polypeptides participate in essential cellular processes.
The recent discovery of numerous human short open reading frame (sORF)-encoded polypeptides (SEPs) has raised important questions about the functional roles of these molecules in cells. Here, we show that a 69-amino acid SEP, MRI-2, physically interacts with the Ku heterodimer to stimulate DNA double-strand break ligation via nonhomologous end joining. The characterization of MRI-2 suggests that this SEP may participate in DNA repair and underscores the potential of SEPs to serve important biological functions in mammalian cells.

• 出版日期2014-4-18