Developing novel methods to search for substrates of protein kinases such as Rho-kinase

作者：Nishioka Tomoki; Shohag Md Hasanuzzaman; Amano Mutsuki; Kaibuchi Kozo^*

来源：Biochimica et Biophysica Acta-Proteins and Proteomics, 2015, 1854(10): 1663-1666.

DOI：10.1016/j.bbapap.2015.03.001

• Protein phosphorylation
• Protein kinase
• Mass spectrometry

Protein phosphoiylation is a major and essential post-translational modification in eukaryotic cells that plays a critical role in various cellular processes. Recent progresses in mass spectrometry techniques have enabled the effective identification and analysis of protein phosphorylation. Mass spectrometry-based approaches in investigating protein phosphorylation are very powerful and informative and can further improve our understanding of protein phosphorylation as a whole, but they cannot determine the upstream kinases involved. We introduce several studies that attempted to uncover the relationships between various kinases of interest and substrates, including two methods we developed: an in vitro approach termed the kinase-interacting substrate screening (KISS) method and an in vivo approach termed the phosphatase inhibitor and kinase inhibitor substrate screening (PIKISS) method. This article is part of a Special Issue entitled: Inhibitors of Protein Kinases.

• 出版日期2015-10