A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes

作者：Aonuma Hiroka; Yoshimura Aya; Kobayashi Tomomi; Okado Kiyoshi; Badolo Athanase; Nelson Bryce; Kanuka Hirotaka^*; Fukumoto Shinya

来源：Experimental Parasitology, 2010, 125(2): 179-183.

DOI：10.1016/j.exppara.2009.12.023

• Vector
• LAMP
• Diagnosis
• Malaria
• Filariasis

Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the SPECT2 gene of Plasmodium berghei and the cytochrome oxidase subunit I gene of Dirofilaria immitis in mosquitoes. This technique could detect as few as 100 P. berghei-infected red blood cell-equivalents or one D. immitis microfilaria. Moreover, individual species of parasites in mosquitoes could be identified when a mixture of fluorescently labeled primer sets was used. These findings suggest that the multiplex LAMP assay is sensitive and specific enough to identify parasite-bearing mosquitoes in areas where several diseases occur simultaneously. This procedure could increase the efficiency and effectiveness of arthropod-borne disease elimination programs.

• 出版日期2010-6