Morphological and functional changes in mycelium and mycorrhizas of Tuber borchii due to heat stress

作者：Leonardi Pamela; Iotti Mirco; Zeppa Sabrina Donati; Lancellotti Enrico; Amicucci Antonella; Zambonelli Alessandra

来源：Fungal Ecology, 2017, 29: 20-29.

DOI：10.1016/j.funeco.2017.05.003

• Bianchetto truffle
• High temperatures
• Mycelium
• Ectomycorrhizas
• Thioredoxin
• Glutaredoxin
• Cell browning
• Sensitive and tolerant strains

Tuber borchii is an ectomycorrhizal ascomycete with a wide ecological range, which forms valuable fruit bodies (truffles). The effect of heat stress on the growth and morphology of ectomycorrhizas and mycelia of 11 T. borchii strains of different geographical and ecological provenance was evaluated. Mycelia and T. borchii-colonized plants were differentially grow at 22 degrees C, 28 C and 34 degrees C. Further, the expression of two genes involved in stress response was also analysed in strains showing a different growth response to the high temperatures. Four out of 11 strains were classified as tolerant to heat stress based on their ability to grow and form mycorrhizas at 28 degrees C as at 22 degrees C. Only one strain seemed to show a high temperature induced quiescence and survived after exposure at 34 degrees C. The expression of the genes considered in this work seems to be related to the level of heat stress tolerance in a strain.

• 出版日期2017-10