A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway

作者：Nishimura Yuya; Takeda Koichi; Ezawa Ryosuke; Ishii Jun; Ogino Chiaki; Kondo Akihiko^*

来源：Journal of Nanobiotechnology, 2014, 12(1): 11.

DOI：10.1186/1477-3155-12-11

• Bio-nanocapsule
• pH-sensitive peptide
• GALA
• Endosomal escape
• HER2
• Drug delivery

Background: An affibody-displaying bio-nanocapsule (Z(HER2)-BNC) with a hepatocyte specificity derived from hepatitis B virus (HBV) was converted into an affibody, Z(HER2), that recognizes HER2 receptors. This affibody was previously reported to be the result of the endocytosis-dependent specific uptake of proteins and siRNA into target cancer cells. To assist the endosomal escape of inclusions, a helper lipid with pH-sensitive fusogenic ability (1,2-dioleoyl-sn-glycero-3-phos phoethanolamine; DOPE) was conjugated with a Z(HER2)-BNC.
Findings: In this study, we displayed a pH-sensitive fusogenic GALA peptide on the surface of a particle in order to confer the ability of endosomal escape to a Z(HER2)-BNC. A GALA-displaying Z(HER2)-BNC purified from yeast uneventfully formed a particle structure. Furthermore, endosomal escape of the particle was facilitated after endocytic uptake and release of the inclusions to the cytoplasm without the cell toxicity.
Conclusion: The genetic fusion of a GALA peptide to the virus-like particle confers the ability of endosomal escape.

• 出版日期2014-4-1