Nitro-linolenic acid is a nitric oxide donor

作者：Mata Perez Capilla; Sanchez Calvo Beatriz; Begara Morales Juan C; Carreras Alfonso; Padilla Maria N; Melguizo Manuel; Valderrama Raquel; Corpas Francisco J; Barroso Juan B^*

来源：Nitric Oxide-Biology and Chemistry, 2016, 57: 57-63.

DOI：10.1016/j.niox.2016.05.003

• Nitro-fatty acids
• Nitro-linolenic acid
• Nitric oxide donor
• Signaling molecule
• Post-translational modifications
• NO-PTM
• Nitroalkylation
• NO release

Nitro-fatty acids (NO2-FAs), which are the result of the interaction between reactive nitrogen species (RNS) and non-saturated fatty acids, constitute a new research area in plant systems, and their study has significantly increased. Very recently, the endogenous presence of nitro-linolenic acid (NO2-Ln) has been reported in the model plant Arabidopsis thaliana. In this regard, the signaling role of this molecule has been shown to be key in setting up a defense mechanism by inducing the chaperone network in plants. Here, we report on the ability of NO2-Ln to release nitric oxide (NO) in an aqueous medium with several approaches, such as by a spectrofluorometric probe with DAF-2, the oxyhemoglobin oxidation method, ozone chemiluminescence, and also by confocal laser scanning microscopy in Arabidopsis cell cultures. Jointly, this ability gives NO2-Ln the potential to act as a signaling molecule by the direct release of NO, due to its capacity to induce different changes mediated by NO or NO-related molecules such as nitration and S-nitrosylation or by the electrophilic capacity of these molecules through a nitroalkylation mechanism.

• 出版日期2016-7-1