Approaches for cytogenetic and molecular analyses of small flow-sorted cell populations from childhood leukemia bone marrow samples

作者：Obro Nina Friesgaard; Madsen Hans Ole; Ryder Lars Peter; Andersen Mette Klarskov; Schmiegelow Kjeld; Marquart Hanne Vibeke^*

来源：Journal of Immunological Methods, 2011, 369(1-2): 69-73.

DOI：10.1016/j.jim.2011.04.007

• Flow-sorting
• Fluorescence in situ hybridization
• PCR
• Minimal residual disease
• Childhood leukemia

Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.

• 出版日期2011-6-30