Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii

作者：Mariga, Alfred Mugambi; Yang, Wen-jian; Mugambi, Dorothy Kemuma; Pei, Fei; Zhao, Li-yan; Shao, Ya-ni; Hu, Qiuhui^*

来源：Journal of the Science of Food and Agriculture, 2014, 94(15): 3152-3162.

DOI：10.1002/jsfa.6665

• Pleurotus eryngii (DC
• ex Fr
• ) Quel protein
• extraction
• characterisation
• antiproliferative activity
• immunomodulatory activity

BACKGROUNDCurrently, the use of mushrooms as functional foods, nutraceuticals or phytopharmaceuticals source has risen. In contrast, the possible cellular cytotoxicity and immunostimulatory activity of Pleurotus eryngii (DC. ex Fr.) Quel protein (PEQP) is unknown. Here we report extraction, anti-tumorigenic and immunostimulatory activity of PEQPin vitro.
RESULTSPEQP was extracted from the dried fruiting bodies of P. eryngii, purified and characterised. Its in vitro antiproliferative activity was then evaluated in human non-small cell lung cancer A549 (NSCLC), stomach adenocarcinoma BGC-823, hepatocellular carcinoma HepG2 and gastric carcinoma HGC-27 cell lines using conventional cancer drugs (paclitaxel, doxorubicin and mitomycin C) as positive controls. The protein fractions (PEQP 1, 2, 3 and 4) obtained inhibited tumour cell proliferation dose-dependently with fraction PEQP 2 having significant (P < 0.05) toxicity in all tumour cells. PEQP had no significant toxicity on normal liver Chang cells but their proliferation was significantly inhibited by mitomycin C. Moreover, PEQP stimulated the proliferation, lysosomal enzyme activity, pinocytosis, nitric oxide and hydrogen peroxide production of RAW 264.7 cell lines dose-dependently.
CONCLUSIONBased on these results, P. eryngii protein has a potential application in functional foods as a natural anti-tumour agent with immunostimulatory activity.

• 出版日期2014-12
• 单位南京农业大学; 南京财经大学