Norovirus genotypes implicated in two oyster-related illness outbreaks in Ireland

作者：Rajko Nenow P^*; Keaveney S; Flannery J; McIntyre A; Dore W

来源：Epidemiology and Infection, 2014, 142(10): 2096-2104.

DOI：10.1017/S0950268813003014

• Molecular epidemiology
• norovirus
• outbreaks
• oysters
• sequencing

We investigated norovirus (NoV) concentrations and genotypes in oyster and faecal samples associated with two separate oyster-related outbreaks of gastroenteritis in Ireland. Quantitative analysis was performed using real-time quantitative reverse transcription polymerase chain reaction and phylogenetic analysis was conducted to establish the NoV genotypes present. For both outbreaks, the NoV concentration in oysters was > 1000 genome copies/g digestive tissue and multiple genotypes were identified. In faecal samples, GII.13 was the only genotype detected for outbreak 1, whereas multiple genotypes were detected in outbreak 2 following the application of cloning procedures. While various genotypes were identified in oyster samples, not all were successful in causing infection in consumers. In outbreak 2 NoV GII.1 was identified in all four faecal samples analysed and NoV GII concentrations in faecal samples were > 10(8) copies/g. This study demonstrates that a range of NoV genotypes can be present in highly contaminated oysters responsible for gastroenteritis outbreaks.

• 出版日期2014-10