A novel transgenic mice model for venous malformation

作者：Wang, Yan An; Zheng, Jia Wei^*; Fei, Zhao Liang; Jiang, Xin Quan; Wang, Zhu Gang; Fei, Jian; Zhang, Zhi Yuan

来源：Transgenic Research, 2009, 18(2): 193-201.

DOI：10.1007/s11248-008-9224-1

• Venous malformation
• Animal model
• Tet-On system
• Transgene
• Murine polyomavirus middle T antigen (PyMT)

Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vascular anomalies in vivo, as well as the study of PyMT's molecular function. A new conditional transgenic vector based on Tet-On system was constructed successfully. After the experiment in vitro, pronuclear microinjection method was used to introduce the purified transgene into the chromosomes of fertilized mice eggs, and five transgenic positive mice were obtained. The transgenic positive animals went down to future generation by hybridization. After induction of PyMT's expression in the F1 generation, three transgenic mice developed venous malformation which was confirmed histopathologically. The mice model generated could be used as a tool to study venous malformation, as well as the function of PyMT gene.

• 出版日期2009-4
• 单位上海市口腔病防治院; 上海交通大学