A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage

作者：Aum Diane J; Vellimana Ananth K; Singh Itender; Milner Eric; Nelson James W; Han Byung Hee; Zipfel Gregory J^*

来源：Scientific Reports, 2017, 7(1): 9126.

DOI：10.1038/s41598-017-09070-y

Various techniques have been developed to study changes in the cerebral vasculature in numerous neuropathological processes including subarachnoid hemorrhage (SAH). One of the most widely employed techniques uses India ink-gelatin casting, which presents numerous challenges due to its high viscosity, rapid solidification, and its impact on immunohistochemical analysis. To overcome these limitations, we developed a novel technique for assessing cerebral vasospasm using cerebrovascular perfusion with ROX, SE (5-Carboxy-X-Rhodamine, Succinimidyl Ester), a fluorescent labeling dye. We found that ROX SE perfusion achieves excellent delineation of the cerebral vasculature, was qualitatively and quantitatively superior to India ink-gelatin casting for the assessment of cerebral vasospasm, permits outstanding immunohistochemical examination of non-vasospasm components of secondary brain injury, and is a more efficient and cost-effective experimental technique. ROX SE perfusion is therefore a novel and highly useful technique for studying cerebrovascular pathology following experimental SAH.

• 出版日期2017-8-22