A modified version of the digestion-ligation cloning method for more efficient molecular cloning

作者：Gao, Song; Li, Yanling; Zhang, Jiannan; Chen, Hongman; Ren, Daming; Zhang, Lijun; An, Yingfeng^*

来源：Analytical Biochemistry, 2014, 453: 55-57.

DOI：10.1016/j.ab.2014.02.031

• Blunt-end DNA ligation
• T4 DNA ligase
• Molecular cloning
• Digestion-ligation approach

Here we describe a modified version of the digestion-ligation approach for efficient molecular cloning. In comparison with the original method, the modified method has the additional steps of gel purification and a second ligation after the first ligation of the linearized vector and DNA insert. During this process, the efficiency and reproducibility could be significantly improved for both stick-end cloning and bluntend cloning. As an improvement of the very important molecular cloning technique, this method may find a wide range of applications in bioscience and biotechnology.

• 出版日期2014-5-15
• 单位沈阳农业大学