Atorvastatin prevents advanced glycation end products (AGEs)-induced cardiac fibrosis via activating peroxisome proliferator-activated receptor gamma (PPAR-gamma)

作者：Chen, Miao; Li, Hongwei^*; Wang, Guoxing^*; Shen, Xuhua; Zhao, Shumei; Su, Wen

来源：Metabolism-Clinical and Experimental, 2016, 65(4): 441-453.

DOI：10.1016/j.metabol.2015.11.007

• Advanced glycation end products
• Atorvastatin
• RAGE
• PPAR-gamma

Background. Previous studies have shown that the activation of advanced glycation end products (AGEs) contributed to the cardiac fibrosis in diabetic patients. Although it had been reported that statins have beneficial effects on cardiac fibrosis in hypertension and myocardial ischemia models, their effects on AGEs models have not been studied. We aimed to investigate the effects of atorvastatin (Ator) on the AGEs-induced cardiac fibrosis both in vitro and vivo.
Methods. Male Sprague-Dawley rats were randomly divided into four groups: Control, AGEs, Ator or AGEs + Ator. The cardiac function was evaluated with the echocardiography at the second and the third month. Fibrosis area, alpha-SMA and RAGE expression in cardiac tissue were measured. For in vitro study, rat cardiac fibroblasts were treated with PD98059 (ERK inhibitor), Ator or Ator + GW9662 (PPAR-gamma antagonist), and then were stimulated with AGEs. Fibroblasts proliferation, ERK1/2, phosphorylated ERK1/2, alpha-SMA, and RAGE expression were studied.
Results. Compared with the control group, in vivo treatment with Ator significantly retarded the AGEs-induced diastolic function and attenuated cardiac fibrosis, alpha-SMA, and RAGE over expression induced by AGEs. Consistently, Ator prominently downregulated RAGE and alpha-SMA, while inhibited phosphorylation of ERK1/2 and fibroblast proliferation induced by AGEs in vitro. The GW9662 neutralized these effects of Ator on cardiac fibroblasts stimulated by AGEs.
Conclusion. In this study, we demonstrated that AGEs-induced fibroblast proliferation and differentiation were dependent on AGEs-RAGE-ERK1/2 pathway and that atorvastatin could block this pathway via activating PPAR-gamma.

• 出版日期2016-4
• 单位首都医科大学