Angiogenic dysfunction in molar pregnancy

作者：Kanter David^*; Lindheimer Marshall D; Wang Eileen; Borromeo Romana G; Bousfield Elizabeth; Karumanchi S Ananth; Stillman Isaac E

来源：American Journal of Obstetrics and Gynecology, 2010, 202(2): 184.e1.

DOI：10.1016/j.ajog.2009.09.005

• antiangiogenic factors
• fms-like tyrosine kinase receptor
• hydatidiform mole
• molar pregnancy
• soluble fms-like tyrosine kinase receptor 1

OBJECTIVE: Molar pregnancy is associated with very early-onset preeclampsia. Since excessive circulating antiangiogenic factors may play a pathogenic role in preeclampsia, we hypothesized that molar placentas produce more antiangiogenic proteins than normal placentas.
STUDY DESIGN: This retrospective case-control study used a semiquantitative immunohistochemical technique to compare histologic sections of molar placentas to normal controls. Tissue slides were treated with 2 antisera: one recognized the antiangiogenic markers fms-like tyrosine kinase receptor 1 (Flt1) and its soluble form (sFlt1), while the other recognized vascular endothelial marker CD31. Stain intensity was graded from 1 + (strong focal staining) to 4 + (91-100% staining).
RESULTS: Molar placentas (n = 19) showed significantly more staining than controls (n = 16) for Flt/sFlt1 (P < .0001).
CONCLUSION: There was a significant difference in Flt1/sFlt1 immunostaining intensity when molar placentas were compared to controls. This supports a hypothesis that the phenotype of preeclampsia in molar pregnancy may result from trophoblasts overproducing at least 1 antiangiogenic protein.

• 出版日期2010-2