Acute Effects of Pyrethroids on Serotonin Release in the Striatum of Awake Rats: An In Vivo Microdialysis Study

作者：Hossain Muhammad M^*; Suzuki Tadahiko; Richardson Jason R; Kobayashi Haruo

来源：Journal of Biochemical and Molecular Toxicology, 2013, 27(2): 150-156.

DOI：10.1002/jbt.21450

• Pyrethroids
• Microdialysis
• Striatum
• Serotonin
• Tetrodotoxin
• Nimodipine

The present study examined the acute neurotoxic effects of three different pyrethroids, allethrin, cyhalothrin, and deltamethrin on the release of serotonin (5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in the striatum of conscious rats using microdialysis. Allethrin 10mg/kg reduced extracellular levels of 5-HT to 46%, whereas 20 and 60mg/kg increased the release to 177% and 243% of baseline, respectively. Cyhalothrin increased 5-HT release to 145204% and deltamethrin decreased to 5832% of baseline in a dose-dependent manner. None of the pyrethroids tested altered extracellular levels of 5-HIAA. Local infusion of the voltage-gated sodium channel antagonist tetrodotoxin (TTX) into striatum completely prevented the effects of allethrin, cyhalothrin, and deltamethrin (10 and 20mg/kg) on 5-HT release. The effect of deltamethrin at 60mg/kg was completely abolished by striatal infusion of nimodipine (L-type Ca++ channel antagonist) with TTX. These findings suggest that pyrethroids disrupt the serotonergic neurotransmission in striatum in a dose-related manner with Na+ and Ca2+ channel-dependent mechanisms.

• 出版日期2013-2