Neuropilin-1 Interacts With the Second Branchial Arch Microenvironment to Mediate Chick Neural Crest Cell Dynamics

作者：McLennan Rebecca; Kulesa Paul M^*

来源：Developmental Dynamics, 2010, 239(6): 1664-1673.

DOI：10.1002/dvdy.22303

• neural crest
• cell migration
• chick
• neuropilins
• confocal
• time-lapse

Cranial neural crest cells (NCCs) require neuropilin signaling to reach and invade the branchial arches. Here, we use an in vivo chick model to investigate whether the neuropilin-1 knockdown phenotype is specific to the second branchial arch (ba2), changes in NCC behaviors and phenotypic consequences, and whether neuropilins work together to facilitate entry into and invasion of ba2. We find that cranial NCCs with reduced neuropilin-1 expression displayed shorter protrusions and decreased cell body and nuclear length-to-width ratios characteristic of a loss in polarity and motility, after specific interaction with ba2. Directed NCC migration was rescued by transplantation of trangfected NCCs into rhombomere 4 of younger hosts. Lastly, reduction of neuropilin-2 expression by shRNA either solely or with reduction of neuropilin-1 expression did not lead to a stronger head phenotype. Thus, NCCs, independent of rhombomere origin, require neuropilin-1, but not neuropilin-2 to maintain polarity and directed migration into ba2. Developmental Dynamics 239:1664-1673, 2010.

• 出版日期2010-6
• 单位河北医科大学