A cis-acting regulatory element that affects the alternative splicing of a muscle-specific exon in the mouse NCAM gene

作者：Kawahigashi H; Harada Y; Asano A; Nakamura M^*

来源：Biochimica et Biophysica Acta-Gene Structure and Expression, 1998, 1397(3): 305-315.

DOI：10.1016/S0167-4781(98)00021-9

• NCAM
• alternative splicing
• myoblast
• fibroblast
• differentiation
• MyoD

The pre-mRNA encoding the neural cell adhesion molecule (NCAM) is spliced to generate NCAM isoforms containing the muscle-specific domain (MSD) during myogenesis. Utilizing chimeric NCAM minigenes, we searched for cis-acting elements that contribute to the alternative selection of exon MSDb, one of the four exons encoding MSD, and identified an intronic cis-element located downstream of exon MSDb. The cis-element acted as a negative regulator for the selection of exon MSDb in nonmuscle fibroblasts but not in myoblasts, that are already destined to differentiate into muscle cells. The suppressive effect of this cis-element on the selection of exon MSDb was released in the process of myogenesis. When MyoD was co-expressed with a minigene containing this element in fibroblasts, the suppressive effect of the cis-element was released as the cells underwent differentiation. We propose that this cis-element contributes at least as one of the regulatory elements in the differentiation state-dependent selection of MSD exons in vivo.

• 出版日期1998-5-11