A novel assay for extracellular matrix remodeling associated with liver fibrosis: An enzyme-linked immunosorbent assay (ELISA) for a MMP-9 proteolytically revealed neo-epitope of type III collagen

作者：Barascuk N^*; Veidal S S; Larsen L; Larsen D V; Larsen M R; Wang J; Zheng Q; Xing R; Cao Y; Rasmussen L M; Karsdal M A

来源：Clinical Biochemistry, 2010, 43(10-11): 899-904.

DOI：10.1016/j.clinbiochem.2010.03.012

• Extracellular matrix remodelling
• Type III collagen
• Matrix-metallo-proteinase-9
• Liver fibrosis
• ELISA

Objectives: Accumulation of extracellular matrix (ECM) components and increased matrix-metalloprotease (MMPs) activity are hallmarks of fibrosis. We developed an ELISA for quantification of MMP-9 derived collagen type III (CO3) degradation.
Design and methods: A monoclonal antibody targeting a specific MMP-9 cleaved fragment of CO3 was used for development of a competitive ELISA. The assay was investigated in serum and tissues from bile duct ligated rats (BDL).
Results: The ELISA showed no cross-reaction with either intact CO3, or other collagens. The intra- and inter-assay CV were below 10%. Liver fibrosis was demonstrated in BDL animals by semi quantitative scoring (P<0.0001). Serum levels of CO3-610 increased 2.5 fold in BDL animals (P<0.001). The CO3-610 levels were 5 fold higher in ex vivo cultures of fibrotic livers compared to controls (P<0.001).
Conclusion: We have developed a novel ELISA for measuring a specific fragment CO3 generated by MMP-9 important in pathogenesis of liver fibrosis.

• 出版日期2010-7