An enhanced strategy using poly(m-aminobenzene sulfonic acid) nanofibres for rapid detection of DNA oxidative damage induced by hollow TiO2 nanocubes

作者：Zhang Wei; Yang Tao; Yin Chuanxia; Li Guicun; Jiao Kui^*

来源：Electrochemistry Communications, 2009, 11(4): 783-786.

DOI：10.1016/j.elecom.2009.01.035

• TiO2 nanocubes
• PABSA nanofibres
• DNA damage
• Co(phen)(3)(3 )

A novel membrane comprising of hollow TiO2 nanocubes and poly(m-aminobenzene sulfonic acid) (PABSA) nanofibres was constructed on the glassy carbon electrode (GCE) for detecting the oxidative damage of natural dsDNA induced by TiO2 under UV radiation. The TiO2/PABSA membrane was used for efficient dsDNA immobilization, dsDNA oxidation through photogenerated hydroxyl radicals, and as the platform for electrochemical sensing. The properties of the TiO2/PABSA membrane and the immobilization of dsDNA were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy using [Fe(CN)(6)](3-/4-) as the indicator. The resulting oxidative damage of dsDNA was detected by monitoring the CV response of an intercalated electroactive probe, namely, Co(phen)(3)(3 ) PABSA has a profound synergistic effect with the hollow TiO2 on the DNA damage detection and the sensor has the potential of becoming a powerful tool for the rapid assessment of the genotoxicity of existing and new chemicals.

• 出版日期2009-4
• 单位青岛科技大学