Development of a bioreactor for the mechanical stimulation of agarose hydrogels

作者：Lee Seung Jae; Park Jeong Hun; Seol Yong Joon; Lee In Hwan^*; Kang Sang Soon; Cho Dong Woo

来源：Microelectronic Engineering, 2009, 86(4-6): 1411-1415.

DOI：10.1016/j.mee.2009.02.026

• Mechanical stimulation
• Scaffold
• Bioreactor
• Agarose

Mechanical stimulation plays a role in improving cell growth in the skeletal system. Many studies have reported the development of bioreactors to stimulate cell-seeded, three-dimensional scaffolds. In this study, we developed a bioreactor capable of applying controlled compression to a cell-seeded agarose hydrogel. This bioreactor consists of a circulation system and compression system. In the circulation system, the culture chamber was sealed to prevent contamination and a pump circulated the medium. In the compression system, mechanical stimuli were controlled by LabVIEW software and a mechanical transfer system. To compare the effects of mechanical stimulation on agarose hydrogel scaffolds, we cultured MC3T3-E1 cells statically and dynamically. The number of cells was increased by 43% at seven days under the intermittent condition than under the static conditions.

• 出版日期2009-6