Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro

作者：Silva e Bastos Matheus; de Souza Luciana Angelo; Onofre Thiago Souza; Silva Junior Abelardo; de Almeida Marcia Rogeria; Bressan Gustavo Costa; Rangel Fietto Juliana Lopes

来源：Memorias do Instituto Oswaldo Cruz, 2017, 112(2): 155-159.

DOI：10.1590/0074-02760160237

• Leishmania braziliensis
• green fluorescent protein
• drug screening

BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening.
OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening.
METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays.
FINDINGS Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin.
MAIN CONCLUSION Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.

• 出版日期2017-2