Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells

作者：Leung Siu Ling; Zha Zhengbao; Cohn Celine; Dai Zhifei; Wu Xiaoyi^*

来源：Colloids and Surfaces B: Biointerfaces , 2014, 121: 141-149.

DOI：10.1016/j.colsurfb.2014.06.011

• Organic inorganic hybrid lipid nanovesicles
• Anti-EGFR antibody
• Selective targeting
• Cancer cells

Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic inorganic hybrid liposomal immunocerasomes via maleimide thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.

• 出版日期2014-9-1
• 单位北京大学