Authenticity and Traceability of Vanilla Flavors by Analysis of Stable Isotopes of Carbon and Hydrogen

作者：Hansen Anne Mette Solvbjerg^*; Fromberg Arvid; Frandsen Henrik Lauritz

来源：Journal of Agricultural and Food Chemistry, 2014, 62(42): 10326-10331.

DOI：10.1021/jf503055k

• isotope ratio mass spectrometry
• stable isotope ratios
• adulteration
• vanillin
• geographic origin
• GC-IRMS

Authenticity and traceability of vanilla flavors were investigated using gas chromatographyisotope ratio mass spectrometry (GC-IRMS). Vanilla flavors produced by chemical synthesis (n = 2), fermentation (n = 1), and extracted from two different species of the vanilla orchid (n = 79) were analyzed. The authenticity of the flavor compound vanillin was evaluated on the basis of measurements of ratios of carbon stable isotopes (delta C-13). It was found that results of delta C-13 for vanillin extracted from Vanilla planifolia and Vanilla tahitensis were significantly different (t test) and that it was possible to differentiate these two groups of natural vanillin from vanillin produced otherwise. Vanilla flavors were also analyzed for ratios of hydrogen stable isotopes (delta H-2). A graphic representation of delta C-13 versus delta H-2 revealed that vanillin extracted from pods grown in adjacent geographic origins grouped together. Accordingly, values of delta C-13 and delta H-2 can be used for studies of authenticity and traceability of vanilla flavors.

• 出版日期2014-10-22