Magnetic Polymer Microspheres with Azidocarbonyl Groups: Synthesis, Characterization and Application in Protein Immobilization

作者：Guo Na; Wu Daocheng^*; Pan Xinghua; Lu Maolin

来源：Journal of Applied Polymer Science, 2009, 112(4): 2383-2390.

DOI：10.1002/app.29755

• magnetic microspheres
• copolymerization
• functionalization of polymers
• azidocarbonyl group
• protein immobilization

A novel magnetic polymer microsphere with amide groups and carboxyl groups was synthesized and reported here. The azidocarbonyl groups were derived from amide groups and linked to the proteins to investigate their immobilization capacity. The morphology, size, functional groups and magnetic properties of magnetic microspheres were characterized by optical microscopy, particle size analyzer, atom force microscopy, magnetic force microscopy, fourier transform infrared spectrometer, vibrating-sample magnetometer and thermal gravimetric analysis. The results indicated that the magnetic polymer microspheres had a well spherical shape with the size ranging from 1 to 10 mu m, highly re-active functional groups, superparamagnetism and strong magnetic responsibility with saturation magnetization of 18.443 emu/g and Fe(3)O(4) content around 21%. The immobilization capacity (eta) was over 70%, The novel azidocarbonyl magnetic polymer microspheres showed potentials to be a good magnetic support and promising applications in bioseparation and biomedical fields.

• 出版日期2009-5-15
• 单位西安交通大学