A metabolic-based approach to improve xylose utilization for fumaric acid production from acid pretreated wheat bran by Rhizopus oryzae

作者：Wang Guanyi; Huang Di; Li Yong; Wen Jianping^*; Jia Xiaoqiang

来源：Bioresource Technology, 2015, 180: 119-127.

DOI：10.1016/j.biortech.2014.12.091

• Wheat bran
• Fumaric acid
• Rhizopus oryzae
• Metabolic profiling
• Xylose

In this work, wheat bran (WB) was utilized as feedstock to synthesize fumaric acid by Rhizopus oryzae. Firstly, the pretreatment process of WB by dilute sulfuric acid hydrolysis undertaken at 100 degrees C for 30 min offered the best performance for fumaric acid production. Subsequently, through optimizing the seed culture medium, a suitable morphology (0.55 mm pellets diameter) of R. oryzae was obtained. Furthermore, a metabolic-based approach was developed to profile the differences of intracellular metabolites concentration of R. oryzae between xylose (the abundant sugar in wheat bran hydrolysate (WBH)) and glucose metabolism. The xylitol, sedoheptulose 7-phosphate, ribulose 5-phosphate, glucose 6-phosphate, proline and serine were responsible for fumaric acid biosynthesis limitation in xylose fermentation. Consequently, regulation strategies were proposed, leading to a 149% increase in titer (up to 15.4 g/L). Finally, by combinatorial regulation strategies the highest production was 20.2 g/L from WBH, 477% higher than that of initial medium.

• 出版日期2015-3
• 单位天津大学; 南开大学