2,6-Bis(4-sulpho-1-hydroxy-2-naphthylazo)pyridine: Spectrophotometric Reagent for the Trace Determination of Zinc in Biological Samples

作者：Ashok Sharma K; Singh Kadyan Partap; Ishwar Singh

来源：Research Journal of Chemistry and Environment, 2012, 16(4): 68-73.

• Chromogenic
• Zinc determination
• Spectrophotometry
• 2,6-bis(4-sulpho-1-hydroxy-2-naphthylazo) pyridine
• sodium salt

A simple, sensitive and highly selective method is proposed for the determination of zinc (II) using a bisazo dye, 2,6-bis(4-sulpho-1-hydroxy-2-naphthylazo) pyridine, sodium salt(PBS) as spectrophotometric reagent. At pH 6.6, in aqueous medium the complex is found to obey Beer's law up to 2.4mg/L with an optimum concentration range between 0.64 to 2.0mg/L. Sandell's sensitivity of the color reaction was calculated to be 0.0012 mu gcm(-2) with molar absorptivity of 5.3x10(4) L. mol(-1)cm(-1) at 560nm. The optimum conditions for the determination of Zn (II) with the reagent were ascertained. The complexation at different pH was studied in aqueous medium. The composition of the complex is 1:2. The action of some interfering ions was verified and the developed method applied successfully for the estimation of zinc levels in biological samples and the results were then compared with those obtained by using AAS.

• 出版日期2012-12