MicroRNA-384 Inhibits the Growth and Invasion of Renal Cell Carcinoma Cells by Targeting Astrocyte Elevated Gene 1

作者：Song, Haitao; Rao, Yanwei; Zhang, Gang; Kong, Xiangbo^*

来源：Oncology Research, 2018, 26(3): 457-466.

DOI：10.3727/096504017X15035025554553

• Astrocyte elevated gene 1 (AEG-1)
• miR-384
• Renal cell carcinoma (RCC)

MicroRNAs (miRNAs) are emerging as pivotal regulators in the development and progression of various cancers, including renal cell carcinoma (RCC). MicroRNA-384 (miR-384) has been found to be an important cancer-related miRNA in several types of cancers. However, the role of miR-384 in RCC remains unclear. In this study, we aimed to investigate the potential function of miR-384 in regulating tumorigenesis in RCC. Here we found that miR-384 was significantly downregulated in RCC tissues and cell lines. Overexpression of miR-384 significantly inhibited the growth and invasion of RCC cells. whereas inhibition of miR-384 had the opposite effects. Bioinformatic analysis and luciferase reporter assay showed that naiR-384 directly targeted the 3'-untranslated region of astrocyte elevated gene 1 (AEG-1). Further data showed that miR-384 could negatively regulate the expression of AEG-1 in RCC cells. Importantly, miR-384 expression was inversely correlated with AEG-1 expression in clinical RCC specimens. Moreover, miR-384 regulates the activation of Wnt signaling. Overexpression of AEG-1 significantly reversed the antitumor effects of miR-384. Overall, these findings suggest that miR-384 suppresses the growth and invasion of RCC cells via downregulation of AEG-1. providing a potential therapeutic target for the treatment of RCC.

• 出版日期2018
• 单位吉林大学; 吉林省人民医院