科研之友
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摘要
Tumor infiltrating lymphocytes (TIL) reflect the host's anti-tumor immune response, and can be a valuable predictor of prognosis. However, many properties of TIL are not fully understood. In the present study, TCR-V beta repertoires of cancer patients were primarily analyzed by flow cytometry. Abnormally expressed TCR-V beta subfamilies were generally found in both TIL and peripheral blood lymphocytes (PBL) of each patient. Of note, increased patient age was associated with increasingly biased TCR-V beta repertoire in TIL but not in PBL, and the dispersion degree of the differences of TCR-V beta subfamilies between TIL and PBL correlated positively with age (P = 0.007). Utilizing immunoscope analysis, we identified the age-related reduction in TCR-V beta diversity, but polyclonal pattern was predominant in significantly expanded TCR-V beta subfamilies. In addition, we found that older patients possessed a decreased ratio of CD8(+)CD62L(+) non-effector cells in TIL compared to PBL, implying age-related increase of CD8(+)CD62L(-) effector cells in TIL. The colocalization analysis of CD8 and CD3, however, suggested the suppressed activity of these effector cells in tumor microenvironment. These findings further elucidate the properties of TIL, showing an increasing difference between TIL and PBL with age, which may provide insight for the development of effective immunotherapies for cancer patients of different ages.
