Nanoarray-surfaces by reconstitution of the porin MspA into stabilized long-chain-lipid-monolayers at a gold-surface

作者：Woerner Michael; Niebler Sandra; Gogritchiani Eliso; Egner Nicole; Braun Andre M; Niederweis Michael; Bossmann Stefan H

来源：Electroanalysis, 2006, 18(19-20): 1859-1870.

DOI：10.1002/elan.200603636

• MspA (porin a from m. smegmatis)
• SAM (self-assembled monolayer)
• CV (cyclic voltammetry)
• EIS (electrochemical impedance spectroscopy)
• Cu(O)-nanoparticles

We have reconstituted the channel porin MspA from M. smegmatis on a gold electrode into a long-chain lipid monolayer resembling a biological membrane. The tailored deposition of the self-assembled monolayer (SAM) on gold was achieved by employing electrochemically initiated generation of thiyl-radicals from organic thiosulfates (Bunte-Salts), which then adsorb onto the gold's surface. At surface coverage of the lipids of approximately 80%, MspA reconstituted efficiently from the aqueous buffer into the SAM. The combination of cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), reflection electron microscopy (REM) and the formation of copper(0)-nanoparticles from copper(II)-ions inside the MspA-channels proved that MspA forms SAM-spanning ion channels on gold, keeping the electrode (surface) accessible through the pores.

• 出版日期2006-10