A modified vitrification method reduces spindle and chromosome abnormalities

作者：Liu, Minghui; Zhou, Wenhui; Chu, Dapeng; Fu, Lei; Sha, Wei; Liu, Shan^*; Li, Yuan^*

来源：Systems Biology in Reproductive Medicine, 2017, 63(3): 199-205.

DOI：10.1080/19396368.2017.1285370

• Human oocyte
• pre-equilibrium temperature
• vitrification

Development of an effective system for oocyte-cryopreservation is of clinical relevance in reproductive medicine. However, oocyte-preservation is not as effective as embryo preservation. In this study, we used a 37 degrees C pre-equilibrium temperature as part of a modified vitrification method for human oocyte cryopreservation. The effect of the new method on spindle configuration, chromosomal arrangement, and mitochondrial distribution was investigated in in vitro-matured human oocytes. A total of 101 in vitro-matured oocytes were randomly assigned for vitrification at pre-equilibrium temperature of 37 degrees C (37 degrees C Group, n=50) or at room temperature (RT Group, 22-24 degrees C, n=51). The time needed for vitrification in the 37 degrees C group was significantly shorter than that in the RT group. Defective spindles were found in 45.5% and 69.0% oocytes in the 37 degrees C group and RT group, respectively (p < 0.05). Abnormal chromosomes were found in 47.7% and 71.4% oocytes, respectively (p < 0.05). There were no significant differences with respect to oocyte survival rate and mitochondrial distribution pattern between the two groups. These results indicate that vitrification at a pre-equilibrium temperature of 37 degrees C may reduce the incidence of defective spindle configuration and chromosomal abnormalities in in-vitro-matured human oocytes.Abbreviations: ICSI: intracytoplasmic sperm injection; FSH: follicle-stimulating hormone; MII: metaphase II; EG: ethylene glycol; PROH: 1,2-propanediol

• 出版日期2017-6
• 单位首都医科大学