Nucleotide sequence of the Schizosaccharomyces pombe lys1(+) gene and similarities of the lys1(+) protein to peptide antibiotic synthetases

作者：Bhattacherjee V; Bhattacharjee JK^*

来源：Yeast, 1998, 14(5): 479-484.

DOI：10.1002/(SICI)1097-0061(19980330)14:5<479::AID-YEA236>3.0.CO;2-T

• lys1(+) gene
• Schizosaccharomyces pombe
• alpha-aminoadipate reductase
• peptide synthetase
• lysine biosynthesis

The 4.2 kbp lys1(+) gene of Schizosaccharomyces pombe encoding the large subunit of alpha-aminoadipate reductase (EC 1.2.1.31), an enzyme specific to lysine synthesis in higher fungi, was completely sequenced at the nucleotide level from pLYS1H. The S. pombe lys1(+) gene product consists of 1415 amino acid residues and has a putative molecular weight of 155.8 kDa. The encoded protein converts alpha-aminoadipic acid to alpha-aminoadipate-delta-semialdehyde by an ATP-mediated adenylation. Analysis of the sequence showed that the putative protein encoded by lys1(+) shares strong homology with the peptide antibiotic synthetases which also use an adenylation step. The sequence data reported in this paper have been submitted to GenBank database (Washington DC, USA) under the Accession Number U15923.

• 出版日期1998-3-30