A rapid method for the determination of rosmarinic acid in Cordia dichotoma seeds using ultrasound-assisted extraction and high-performance liquid chromatography-tandem mass spectrometry

作者：Liu, Feng; Tian, Shuge^*

来源：Research Journal of Chemistry and Environment, 2013, 17(12): 145-152.

• C. dichotoma seeds
• Ultrasound-assisted extraction
• Rosmarinic acid
• HPLC-MS/MS
• Single-factor experiments
• Orthogonal experiment

An ultrasound-assisted extraction (UAE) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method that offers good linearity, accuracy and precision was developed for the simultaneous determination of rosmarinic acid (RA) in Cordia dichotoma seeds. Single-factor and orthogonal experiments were performed to determine the appropriate range of extraction conditions and to obtain the optimum extraction conditions respectively. The optimum UAE conditions for C. dichotoma seeds were as follows: ethanol concentration of 70%, solid/liquid ratio of 1: 35 g/mL, ultrasound time of 30 min and ultrasound power of 300 W. UAE is an alternative technique for the fast extraction of C. dichotoma seeds. The factors selected in this study had significant functions on the extraction of RA from C. dichotoma seeds.

• 出版日期2013-12
• 单位新疆师范大学; 新疆医科大学