A Gene Expression Profile Test for the Differential Diagnosis of Ovarian Versus Endometrial Cancers

作者：Lal Anita^*; Panos Rebecca; Marjanovic Mira; Walker Michael; Fuentes Eloisa; Kapp Daniel S; Henner W David; Buturovic Ljubomir J; Halks Miller Meredith

来源：Oncotarget, 2012, 3(2): 212-223.

DOI：10.18632/oncotarget.450

• gene expression
• tissue of origin
• endometrial cancer
• ovarian cancer
• diagnostic test

We have developed a gene expression profile test (Pathwork Tissue of Origin Endometrial Test) that distinguishes primary epithelial ovarian and endometrial cancers in formalin-fixed, paraffin-embedded (FFPE) specimens using a 316-gene classification model. The test was validated in a blinded study using a pre-specified algorithm and microarray files for 75 metastatic, poorly differentiated or undifferentiated specimens with a known ovarian or endometrial cancer diagnosis. Measures of test performance include a 94.7% overall agreement with the known diagnosis, an area under the ROC curve (AUC) of 0.997 and a diagnostic odds ratio (DOR) of 406. Ovarian cancers (n=30) gave an agreement of 96.7% with the known diagnosis while endometrial cancers (n=45) gave an agreement of 93.3%. In a precision study, concordance in test results was 100%. Reproducibility in test results between three laboratories was 94.3%. The Tissue of Origin Endometrial Test can aid in resolving important differential diagnostic questions in gynecologic oncology.

• 出版日期2012-2