科研之友
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摘要
The effluents from the anaerobic methane reactor were characterized by the EEM fluorescence spectra. The EEM fluorescence spectra and relative concentration of the main components were acquired by PARAFAC models. Results show that the main components of the effluents from the uninhibited reactor were protein, coenzyme NADH and F(420). When the methane-producing reaction was inhibited, the production rate of methane decreased rapidly. Correspondingly, the fluorescence of F(420) disappeared and the main components of the reactor were protein and NADH. The fluorescence intensity scores of protein from the diluted sample were remarkably correlated to the protein determination by chemical analysis and the correlation efficient was 0.93. The fluorescence intensity scores of F(420) were correlated to the production rate of methane, which implicated that coenzyme F(420) could be used to reflect the activity of methane production in a certain bioreactor. This work provides a new method to monitor the operation status of anaerobic methane-producing reactor.
