Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin beta subunit gene from Arthrospira platensis by site-directed mutagenesis

作者：Guo Nan; Zhang Xuecheng^*; Lu Yongzhong; Song Xiaojin

来源：Biotechnology Letters, 2007, 29(3): 459-464.

DOI：10.1007/s10529-006-9266-5

• Arthrospira platensis
• cpcB gene
• green fluorescent protein
• site-directed mutagenesis

Six promoters in the 419 bp upstream sequence of the phycocyanin beta subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined.

• 出版日期2007-3
• 单位中国海洋大学; 青岛科技大学