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摘要
A simple and reproducible UPLC-MS/MS method for the determination of itraconazole (ITZ) and its photodegradation products formed during exposure to UV-A radiation was developed. Chromatographic separations were carried out using an Acquity UPLC BEH C-18 column (2.1x100mm, 1.7m particle size). The column was maintained at 40 degrees C, and eluted under gradient conditions from 100% to 50% of eluent A over 13 min, at a flow rate of 0.3mL min(-1). Eluent A was 0.1% (v/v) formic acid in water; eluent B was 0.1% (v/v) formic acid in acetonitrile. The linear regression analysis for the calibration curve showed a good linear correlation over the concentration range 0.0066-0.15mg mL(-1) with determination coefficient>0.99. The activities of some photocatalysts during degradation process of ITZ were compared. It was found that indirect photodegradation of ITZ was more effective than direct photolysis. Under our experimental conditions the photodegradation rate constant depended on the applied catalysts with catalytic activity decreasing in the following pattern: FeCl3>TiO2/FeCl3>TiO2. The kinetic analysis of the photodegradation data revealed that the degradation of the ITZ follows first-order kinetics. The photodegradation products of ITZ were identified, and their fragmentation pathways, derived from MS/MS data, were proposed.
- 出版日期2016-11
