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摘要
A rapid and convenient ultra high performance liquid chromatography. tandem mass spectrometry (UPLC-MS/MS) method for analysis of ribavirin and its two main kinds of metabolites, 1,2,4. triazole-3-carboxamide (TCONH2) and 1 beta D-ribofuranosyl-1,2,4-triazole-3-carboxylic acid (RTCOOH), in fresh eggs has been developed and validated. The sample was extracted with acetonitrile water (9. 1, V /V), added N. hexane to move liposoluble substances, after centrifugation, the upper solvent was cleaned. up by C-18 and GCB, then determined by UPLC-MS/MS coupled with Agilent ZORBAX SB. Aq column (100 mmx 3. 0 mm, 1.8 mu m). Over the concentration range of 2. 0-200. 0 mu g/L (0. 5-200. 0 mu g/L and 5. 0-200. 0 mu g/L) for ribavirin (TCONH2 and RTCOOH) in fresh egg matrix, the correlation coefficients (R-2) of the calibration curves were above 0. 99, and the limits of detection (LODs) were 0. 54 mu g/L (0. 09 and 1. 54 mu g/L). The limits of quantitation (LOQs) were 1. 79, 0. 31 and 5. 13 mu g/L, respectively. At three spiked concentration levels of 5. 0, 10. 0 and 50. 0 mu g/L, the recoveries of ribavirin and RTCOOH ranged from 96. 1% to 99. 6% and 42. 9% to 58. 3%. And at three spiked concentration levels of 0. 5, 2. 0 and 5. 0 mu g/L, the recoveries of TCONH2 ranged from 75. 9% to 106. 7%. The results of the determination in various real samples showed that the method is simple, accurate, rapid and suitable determination of ribavirin and its two main kinds of metabolites in fresh eggs.
- 出版日期2016-6
- 单位中国农业科学院农业质量标准与检测技术研究所; 南京农业大学
