Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium majus (Papaveraceae)

作者：Cahlikova Lucie^*; Opletal Lubomir; Kurfurst Milan; Macakova Katerina; Kulhankova Andrea; Host'alkova Anna

来源：Natural Product Communications, 2010, 5(11): 1751-1754.

• Chelidonium majus
• Papaveraceae
• isoquinoline alkaloids
• Alzheimer s disease
• red blood cells
• acetylcholinesterase
• butyrylcholinesterase
• 6-ethoxydihydrosanguinarine
• 6-ethoxydihydrochelerythrine

The roots and aerial parts of Chelidonium majus L were extracted with EtOH and fractionated using CHCl(3) and EtOH Repeated column chromatography, preparative TLC and crystallization led to the isolation of five isoquinoline alkaloids stylopine (3), chelidonine (4), homochelidonine (5), protopine (6), and allocryptopine (7), along with two isolation artifacts 6-ethoxydihydrosanguinarine (1) and 6-ethoxydihydrochelerythrine (2) All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity The isolation artifacts exhibited the highest activity against HuAChE and HuBuChE with IC(50) values of 0 83 +/- 0 04 mu M and 4 20 +/- 0 19 mu M for 6-ethoxydihydrochelerythrine and 3 25 +/- 0 24 mu M and 4 51 +/- 0 31 mu M for 6-ethoxydihydrosanguinarine The most active of the naturally-occurring alkaloids was chelidonine, which inhibited both HuAChE and HuBuChE in a dose-dependent manner with IC(50) values of 26 8 +/- 1 2 mu M and 31 9 +/- 1 4 mu M, respectively

• 出版日期2010-11