Micropilot: automation of fluorescence microscopy-based imaging for systems biology

作者:Conrad Christian; Wuensche Annelie; Tan Tze Heng; Bulkescher Jutta; Sieckmann Frank; Verissimo Fatima; Edelstein Arthur; Walter Thomas; Liebel Urban; Pepperkok Rainer*; Ellenberg Jan
来源:Nature Methods, 2011, 8(3): 246-U89.
DOI:10.1038/NMETH.1558

摘要

Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.

  • 出版日期2011-3