Differential susceptibility of Hyalomma excavatum adults and nymphs to the entomopathogens Metarhizium anisopliae ARSEF 4556 and Steinernema carpocapsae

作者：Butt Tariq M; Wood Martyn; Taylor James W D; Bakirci Serkan; Hazir Canan; Ulug Derya; Hazir Selcuk^*

来源：International Journal of Pest Management, 2016, 62(3): 261-266.

DOI：10.1080/09670874.2016.1181287

• Hyalomma anatolicum excavatum
• metarhizium anisopliae
• steinernema carpocapsae
• biological control
• ticks

This study investigated the virulence of the entomopathogenic fungus, Metarhizium anisopliae strain ARSEF 4556, and the entomopathogenic nematode Steinernema carpocapsae against adult and nymphal stages of the tick Hyalomma anatolicum excavatum. The fungus was tested at three different concentrations, 10(6), 10(7), and 10(8) conidia ml(-1). Ticks were exposed to 4000 IJs of S. carpocapsae in 0.5ml distilled water in Petri dishes lined with 2 layers of moist filter paper. Adult ticks were not susceptible to M. anisopliae, whereas the nymphs were highly susceptible. Nymph mortality was dose dependent with all ticks being killed at the highest dose 96hours post-inoculation. In contrast, S. carpocapsae was much more effective against engorged adults than unfed adults or nymphs. Tick susceptibility was not influenced by gender. The mean mortalities of the unfed adult males, females, and nymphs were 30% +/- 9.2%, 40% +/- 7.5%, and 36% +/- 4.7%, respectively. Nymphal mortality was 36% and there was a significant difference between nematode treatment and control group (P < 0.05).

• 出版日期2016-9