A novel translational control mechanism involving RNA structures within coding sequences

作者：Jungfleisch Jennifer; Nedialkova Danny D; Dotu Ivan; Sloan Katherine E; Martinez Bosch Neus; Bruening Lukas; Raineri Emanuele; Navarro Pilar; Bohnsack Markus T; Leide Sebastian A; Diez Juana^*

来源：Genome Research, 2017, 27(1): 95-106.

DOI：10.1101/gr.209015.116

The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here, we identify a novel and highly conserved mechanism of translational control involving RNA structures within coding sequences and the DEAD box helicase Dhhl. Using yeast genetics and genome-wide ribosome profiling analyses, we show that this mechanism, initially derived from studies of the Brome Mosaic virus RNA genome, extends to yeast and human mRNAs highly enriched in membrane and secreted proteins. All Dhhl-dependent mRNAs, viral and cellular, share key common features. First, they contain long and highly structured CDSs, including a region located around nucleotide 70 after the translation initiation site; second, they are directly bound by Dhhl with a specific binding distribution; and third, complementary experimental approaches suggest that they are activated by Dhhl at the translation initiation step. Our results show that ribosome translocation is not the only unwinding force of CDS and uncover a novel layer of translational control that involves RNA helicases and RNA folding within CDS providing novel opportunities for regulation of membrane and secretome proteins.

• 出版日期2017-1