Antiviral Effect of Flavonol Glycosides Isolated from the Leaf of Zanthoxylum piperitum on Influenza Virus

作者：Ha Song Yi; Youn Hana; Song Chang Seon; Kang Se Chan; Bae Jong Jin; Kim Hee Tae; Lee Kwang Min; Eom Tae Loon; Kim In Su; Kwak Jong Hwan^*

来源：Journal of Microbiology, 2014, 52(4): 340-344.

DOI：10.1007/s12275-014-4073-5

• Zanthoxylum piperitum
• Rutaceae
• flavonol glycoside
• anti-influenza activity
• neuraminidase inhibitor

The ethanol extract of Zanthoxylum piperitum (L.) DC. showed in vitro antiviral activity against influenza A virus. Three flavonol glycosides were isolated from the EtOAc fraction of Z. piperitum leaf by means of activity-guided chromatographic separation. Structures of isolated compounds were identified as quercetin 3-O-beta-D-galactopyranoside (1), quercetin 3-O-alpha-L-rhamnopyranoside (2), kaempferol 3-O-alpha-L-rhamnopyranoside (3) by comparing their spectral data with literature values. The anti-influenza viral activity of isolates was evaluated using a plaque reduction assay against influenza A/NWS/33 (H1N1) virus. The compounds also were subjected to neuraminidase inhibition assay in influenza A/NWS/33 virus. Compounds 1-3 exhibited antiviral activity against an influenza A virus in vitro, and inhibited the neuraminidase activity at relatively high concentrations.

• 出版日期2014-4